Research Interest:

High efficient separation and characterization of complex samples Selected Projects Undertaken:

1. National Basic Research Program of China (S973 project), 2012CB910600, Research on Novel Methods and Related Techniques for Protein Quantitation, 2012.01-2016.08, 27.5 million

2. Subproject of The Major Program of National Natural Science Foundation, 21190043, Novel aptamer based affinity enrichment and separation methods, 2012.01-2016.12, 3.5 million RMB

3. National Natural Science Foundation for Equipment, 21027002, Research and development on multilevel protein sample pretreatment, 2011.01-2013.12, 2 million RMB

4. Knowledge Innovation Program of Chinese Academy of Sciences, K2010A12, the application of novel technologies and methods for proteome separation and identification in the early detection of tumor metastatis, 2010.07-2012.06, 0.8 million RMB

Prof. Dr. Lihua Zhang, born on Sep. 25, 1973, obtained her Bachelor degree of Science from Department of Chemistry, Jilin University, in 1995. She pursued her Ph.D. study in National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS) under the direction of Prof. Yukui Zhang, Member of CAS. During her graduate study, she was selected to join the scientific exchange program of DAAD, and spent one year in GSF-National Research Center for Environment and Health, Germany, under the direction of Prof. Dr. A. Kettrup. In 2000, she obtained her Ph.D. degree from DICP, CAS. From 2001 to 2003, she carried out the postdoctoral research in Prof. Yoshinobu Baba’s group in Japan. In April 2003, she went back to work in DICP, CAS, as an associate professor. Now she is the group leader of Laboratory of “High efficient separation and high accuracy characterization of biomolecules” in DICP, CAS.

Prof. Dr. Lihua Zhang was selected as "Young and middle-aged leading scientists, engineers and innovators" of Ministry of Science and Technology (2012). She won the Second Prize of National Natural Science (rank second, 2012), First Prize of Natural Science in Liaoning Province (rank second, 2011; rank third, 2004), First Prize of China Association for Instrument Analysis (Rank second, 2006), Distinguished Lectureship Award from the Chemistry Society of Japan (2008) and Young Scientists Award from the Chinese Chemistry Society (2008). Furthermore, she was appointed the Regional Editor China for Analytical and Bioanalytical Chemistry, A-page editor for Analytical Chemistry, Editorial board member for Proteomics, Scientific Report, and Chinese Journal of Chromatography.

Prof. Dr. Lihua Zhang’s research is focused on the new materials, methods and techniques for both qualitative and quantitative proteome analysis. She has hosted or participated in 20 scientific projects organized by National Natural Science Foundation, Ministry of Science and Technology and Chinese Academy of Sciences. Up till now, she has published over 100 papers in journals indexed by SCI. She has been invited to give more than 10 presentations in international scientific conferences.

Selected papers:

1. Bo Jiang, Yu Liang, Qi Wu, Hao Jiang, Kaiguang Yang, Lihua Zhang*, Zhen Liang, Xiaojun Peng, Yukui Zhang, New GO-PEI-Au-L-Cys ZIC-HILIC, composites: synthesie and selective enrichment of glycopeptides, Nanoscale, 2014, 6: 5616-5619.

2. Jianxi Liu, Yanyan Qu, Kaiguang Yang, Qi Wu, Yichu Shan, Lihua Zhang*, Zhen Liang, Yukui Zhang, Monodisperse Boronate Polymeric Particles Synthesized by a Precipitation Polymerization Strategy: Particle Formation and Glycoprotein Response from the Standpoint of the Flory-Huggins Model, Acs Applied Materials & Interfaces, 2014, 6: 2059-2066.

3. Qun Zhao, Yu Liang, Huiming Yuan, Zhigang Sui, Qi Wu, Zhen Liang, Lihua Zhang*, Yukui Zhang, Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion, Analytical Chemistry, 2013, 85, 8507-8512.

4. Yuan Zhou, Yichu Shan, Qi Wu, Shen Zhang, Lihua Zhang*, Yukui Zhang, Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification, Analytical Chemistry, 2013, 85, 10658-10663.

5. Qun Zhao, Yu Liang, Huiming Yuan, Zhigang Sui, Qi Wu, Zhen Liang, Lihua Zhang*, Yukui Zhang, Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion, Analytical Chemistry, 2013, 85, 8507-8512.

6. Yuan Zhou, Yichu Shan, Qi Wu, Shen Zhang, Lihua Zhang*, Yukui Zhang, Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification, Analytical Chemistry, 2013, 85, 10658-10663.

7. Liyuan Zhang, Qun Zhao, Zhen Liang, Yang Kaiguang, Sun Liangliang, Lihua Zhang*, Yukui Zhang, Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides, Chemical Communications, 2012, 48(50): 6274-6276.

8. Yanan Qu, Jianxi Liu, Kaiguang Yang, Zhen Liang, Lihua Zhang*, Yukui Zhang, Boronic Acid Functionalized Core-Shell Polymer Nanoparticles Prepared by Distillation Precipitation Polymerization for Glycopeptide Enrichment, Chemistry-a European Journal, 2012, 18(29): 9056-9062.

9. Huiming Yuan, Yuan Zhou, Simin Xia, Lihua Zhang*, Xiaodan Zhang, Qi Wu, Zhen Liang, Yukui Zhang, Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion, Analytical Chemistry, 2012, 84(11): 5124-5132.

10. Junfeng Ma, Lihua Zhang*, Zhen Liang, Yichu Shan, Yukui Zhang, Immobilized enzyme reactors in proteomics, Trac-Trends in Analytical Chemistry, 2011, 30(5), 691-702.

11. Yanyan Qu, Simin Xia, Huiming Yuan, Qi Wu, Man Li, Lijuan Zou, Lihua Zhang*, Zhen Liang, Yukui Zhang, Integrated Sample Pretreatment System for N-Linked Glycosylation Site Profiling with Combination of Hydrophilic Interaction Chromatography and PNGase F Immobilized Enzymatic Reactor via a Strong Cation Exchange Precolumn, Analytical Chemistry, 2011, 83(19), 7457-7463.

12. Jinxiang Liu, Kaiguang Yang, Qiliang Deng, Qinran Li, Lihua Zhang*, Zhen Liang, Yukui Zhang, Preparation of a new type of affinity materials combining metal coordination with molecular imprinting, Chemical Communications, 2011, 47(13), 3969-3971.

13. Liangliang Sun, Jun Ma, Xiaoqiang Sun, Dingyin Tao, Yanyan Zhang, Yichu Shan, Zhen Liang, Lihua Zhang*, Yukui Zhang, Intergrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion, Analytical Chemistry, 2010, 82(6), 2574-2579.

14. Junfeng Ma, Chunyan Hou, Liangliang Sun, Dingyin Tao, Yanyan Zhang, Yichu Shan, Zhen Liang, Lihua Zhang* , Ling Yang, Yukui Zhang, Coupling Formic Acid Assisted Solubilization and online Immobi-lized Pepsin Microreactor Digestion with SCX-μPRLC-ESI-MS/MS for Integral Membrane Proteome Analysis, Analytical Chemistry, 2010, 82, 9622-9625.

Transferred patents :

1. Lihua Zhang (the first inventor) et al., A kind of capillary monolithic column and its applications in the immobilization of trypsin, 2013.5, China, ZL201010522957.4

2. Lihua Zhang (the first inventor) et al., A type of method for the preparation of porous monolithic material based immobilized enzyme microreactor,2012.3, China, ZL200810010347.9

3. Lihua Zhang (the first inventor) et al., Sample preprocessing device and its preparation for protein collection and enzymatic digestion, 2012.4, China, ZL200710159293.8

4. Lihua Zhang (the first inventor) et al., the device for the preparation of capillary electrospray interface used in Liquid chromatography-MS, 2012.5, China, ZL200710012718.2

5. Lihua Zhang (the first inventor) et al., the method and device for the online injection of large volume sample in microflow liquid chromatography, 2011.8, China, ZL200610134978.2

6. Lihua Zhang (the first inventor) et al., a type of multi-channel electrospray ion source for coupling of micro liquid separation system and mass spectrometry, 2012.4, China, ZL200610134979.7

7. Lihua Zhang (the first inventor) et al., System for the online desalting, enrichment and coupling of double split flow nanoliter scale liquid chromatography with mass spectrometry, 2011.4, China, ZL200610134981.4