Proteomics, liquid chromatography coupled with mass spectrometry analysis, protein quantification, protein Interaction        

1. Development of ionic liquid based membrane protein sample pretreatment method to significantly improve the qualitative and quantitative coverage depth of proteome.

2. Development of in-vivo chemical cross-linking based intracellular protein complex structural analysis method to provide the key technology for realizing spatio-temporal dynamic interaction and functional analysis of proteins in living cell.

Professor, Dalian Institute of Chemical Physics, CAS, 2022/07 - present

Associate Professor, Dalian Institute of Chemical Physics, CAS, 2016/07 - 2022/06

Assistant Professor, Dalian Institute of Chemical Physics, CAS, 2014/07 - 2016/06

Ph.D, Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 2008/09 - 2014/05

B.S., Chemistry, Northwest University, 2004/09 - 2008/07

Dalian Young Science and Technology Star, 2018

Mmember of Youth Innovation Promotion Association of Chinese Academy of Science, 2020

1. Gao, H.; Zhao, L.; Zhong, B.; Zhang, B.; Gong, Z.; Zhao, B.; Liu, Y.; Zhao, Q.*; Zhang, L.*; Zhang, Y., In-Depth In Vivo Crosslinking in Minutes by a Compact, Membrane-Permeable, and Alkynyl-Enrichable Crosslinker. Anal. Chem. 2022, 94 (21), 7551-7558.

2. An, Y.; Zhao, Q.*; Gao, H.; Zhao, L.; Li, X.; Zhang, X.; Liang, Z.; Zhang, L.*; Zhang, Y., Selective Removal of Unhydrolyzed Monolinked Peptides from Enriched Crosslinked Peptides To Improve the Coverage of Protein Complex Analysis. Anal. Chem. 2022, 94 (9), 3904-3913.

3. Chu, H.#; Zhao, Q.#, *; Shan, Y.; Zhang, S.; Sui, Z.; Li, X.; Fang, F.; Zhao, B.*; Zhong, S.; Liang, Z.; Zhang, L.*; Zhang, Y., All-Ion Monitoring-Directed Low-Abundance Protein Quantification Reveals CALB2 as a Key Promoter in Hepatocellular Carcinoma Metastasis. Anal. Chem. 2022, 94 (16), 6102-6111.

4. An, Y.; Zhao, Q.*; Gong, Z.; Zhao, L.; Li, Y.; Liang, Z.; Zou, P.; Zhang, Y.; Zhang, L.*, Suborganelle-Specific Protein Complex Analysis Enabled by in Vivo Cross-Linking Coupled with Proximal Labeling. Anal. Chem. 2022, 94 (35), 12051-12059.

5. Gao, H.; Zhao, Q.*; Gong, Z.; Zhong, B.; Chen, J.; Sui, Z.; Li, X.; Liang, Z.; Zhang, Y.; Zhang, L.*, Alkynyl-Enrichable Carboxyl-Selective Crosslinkers to Increase the Crosslinking Coverage for Deciphering Protein Structures. Anal. Chem. 2022, 94 (36), 12398-12406.

6. Chu, H.#; Zhao, Q.#; Liu, J.; Yang, K.; Wang, Y.; Liu, J.; Zhang, K.; Zhao, B.*; He, H.; Zheng, Y.; Zhong, S.; Liang, Z.; Zhang, L.*; Zhang, Y., Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Anal. Chem. 2022, 94 (2), 758-767.

7. Fang, F.#; Zhao, Q.#; Chu, H.#; Liu, M.; Zhao, B.; Liang, Z.; Zhang, L.*; Li, G.; Wang, L.*; Qin, J.; Zhang, Y., Molecular Dynamics Simulation-assisted Ionic Liquid Screening for Deep Coverage Proteome Analysis. Mol. Cell. Proteomics 2020, 19 (10), 1724-1737.