Time: 10:00 am on Jul. 13th, 2015
Location: Small meeting room of biotechnology building
Reporter: Dr. Liangliang Sun, research assistant professor at University of Notre Dame
Resume：
Dr. Liangliang Sun obtained his bachelor's degree in Bioengineering at University of Dalian Technology in 2005, and his Ph.D. in Analytical Chemistry at Dalian Institute of Chemical Physics in 2011. Prior to joining the Notre Dame faculty as a research assistant professor in 2013, he worked with Professor Norman J. Dovichi at University of Notre Dame as a postdoctoral research fellow from April 2011 to December 2012. His research is focused on the development of high efficient sample preparation methods, high efficient separation methods and high sensitive mass spectrometry detection system for biological molecules. In recent two years, he began to carry our research on the development proteomics taking Xenopus laevis as the animal model. He has published more than 20 articles in scientific journals such as Angew. Chem. Int. Ed., Anal. Chem., Curr. Opin. Chem. Biol. and Sci. Rep. as the first author and more than 50 articles as the first or co-author. A patent of him has been commercialized.
Introduction：
Application of capillary zone electrophoresis-mass spectrometry technique in the characterization of biological molecules especially in the area of proteomics has gained much attention. Prof. Dovichi's group invented a kind of electro-kinetically pumped sheath flow interface, which was further improved to increase the sensitivity and stability. Based on the improved sheath flow interface, the capillary electrophoresis-MS technique was applied to the high sensitive and high throughput characterization of biological molecules. In comparison to the traditional LC-MS system, the detection sensitivity could be increased by one order of magnitude using the developed capillary electrophoresis-MS system, which has the ability for large scale proteomics analysis.
Quantitative proteomics methods based on isotope labeling and multi-dimensional chromatography (LC)-MS/MS was applied to the study of development proteomics. Taking Xenopuslaevis as the model animal, the expression kinetics of proteome during early development of embryos (from fertilized egg to early tailbud) was studied. The data clearly reflect the major events that mark changes in gene expression patterns during early Xenopus development, which provides important evidence for the understanding of embryo early development.
Contact：Prof. Lihua Zhang of group 1810（9720）