Time: 9:00 Nov. 29, 2014
Location: Small Conference Room of Biological Building
Reporter: Prof. Cheng Lin
Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA 02118
Speaker:
Prof. Cheng Lin is Research Assistant Professor of Biochemistry, Department of Biochemistry, Boston University of Medicine. He obtained his Ph. D degree from Cornell University in 2003. As postdoctoral research associate, he conducted his career in Department of Chemistry and Chemical Biology, Cornell University from 2003 to 2004 and Mass Spectrometry Resource and Cardiovascular Proteomics Center, Boston University of Medicine from 2004 to 2009. Then, he became the assistant director of Mass Spectrometry Resource, Boston University of Medicine in 2009. By now, he has published more than 30 articles in famous scientific journals including Science, PNAS, JCAS and Mol. Cell. Proteomics and written a chapter in "Protein and Peptide Mass Spectrometry in Drug Discovery" published by John Wiley & Sons, Inc.
Abstract:
The structural complexity and diversity of glycans parallel their multilateral functions in living systems. To better understand the vital roles glycans play in biological processes, it is imperative to develop analytical tools that can provide detailed glycan structural information.Traditionally, this was achieved by multistage tandem mass spectrometry (MSn) using collision-induced dissociation (CID) as the fragmentation method. However, the MSn approach lacks the sensitivity and throughput needed to analyze complex glycan mixtures from biological sources, often available in limited quantities.Recently, we have demonstrated the utility of electron activated dissociation (ExD) methods in glycan structural analysis. Here, we will present our current understanding of the various ExD processes, as well as their application to analysis of biological samples. We will also present our recent work on the coupling of glycan separation techniques to tandem MS analysis, with emphasis on the development of selected accumulation ion mobility spectrometry (SAIMS)-ExD tandem MS for structural analysis of isomeric glycans.
Contact: 1810 group, Prof. Lihua Zhang (84379720)