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Lecture: Principle and Application, Leica’s STED and GSD super-resolved fluorescence microscopy

Posted: 2014-11-13

TimeNovember 13, 2014(Thursday) 14:00-16:00.

LocationConference Room of Biological building   

Lecturer: Lijun Wu PhD. 

 Senior National Application Manager – Life Science, Leica Microsystems Trading Limited Shanghai 

  

Abstract: 

    For a long time optical microscopy was held back by a presumed limitation – Abbe’s stipulated limit: that it would never obtain a better resolution than half the wavelength of light. In recent decades, however, scientists have achieved excellent results in this area, breaking the optical diffraction limit of resolution. Helped by STED and Molecular Localization Microscopy technique, in what has become known as nanoscopy,scientists visualize the pathways of individual molecules inside living cells.They can see how molecules create synapses between nerve cells in the brain; they can track proteins involved in Parkinson’s, Alzheimer’s and Huntington’s diseases as they aggregate; they follow individual proteins in fertilized eggs as these divide into embryos. It opens the view of scientists in the true sense of vision.Today, nanoscopy is used world-wide and new knowledge of greatest benefit to mankind is produced on a daily basis. It has been Leica Microsystems’ privilege to be able to work and collaborate with Stefan for over 15 years, who is awarded the Nobel Prize in Chemistry 2014. As a result of this collaboration, Leica Microsystems has had the opportunity to exclusively develop commercial versions of the STED microscope for the imaging community. The STED can reach the XY resolution up to 50nm, and the GSD can reach XY resolution up to 20nm. The STED and GSD principle and application in life science will be introduced in this lecture. 

  

Brief introduction of the lecturer: 

    Dr. Lijun Wu was graduated from Shanghai No.2 Medical University in 1985. He got his doctorate in cell biology in the university. He had worked as associate professor in Dept. Histology & Embryology in Shanghai No.2 Medical University. Dr. Wu used to work in Dept. Physiology, Hong Kong Chinese University as professor in International Epithelium Research Association. In 2008, he acted as Regional Manager China, Bitplane AG. Since 2011, he has acted as Senior National Application Manager – Life Science, Leica Microsystems Trading Limited Shanghai. Dr. Wu used to be editorial board member of Chinese Journal of Anatomy; member of the expert committee of reproductive toxicology, Chinese Society of Toxicology; and guest professor in Medical school, Shanghai Jiaotong University. His majority was reproductive biology, reproductive physiology and cell biology. He had been responsible for several National Science FoundationProjects, National Key Projects and some State and Ministry projects. 

  

ContactGroup 18T2 Wenting Yin (89648)