High-cost viral nucleic acid detection devices are limited resources for developing counties and rural areas, leading to underdiagnosis or even pandemics of viral infectious diseases.
Paper-based detection which can provide a point-of-care testing platform for convenient and inexpensive sensors has gained attention in the detecting of many small molecule analytes.
Recently, a research group led by Prof. FENG Liang collaborated with Prof. LIU Yu's group from the Dalian Institute of Chemical Physics (DICP) of the Chinese Academy of Science proposed a novel virus detection strategy enabled by TR512-peptide-based bioorthogonal capture of the functionalized paper. With a home-developed full-set virus detection device, this platform achieved a rapid screen of viral infection.
This study was published in Analytical Chemistry on March 29.
This novel detection method was enabled by TR512-peptide based biorthogonal capture and enrichment of commercially available Texas red fluorophore labeled nucleic acid on the functionalized paper. It was generally applicable to different nucleic acid preamplification strategies (PCR, RAA, CRISPR) and different virus types (HBV, ASFV, HPV16, HPV 18 etc.).
Moreover, they developed a full-set virus detection device in house to detect the presence of the Hepatitis B virus (HBV) viral gene in patients’ blood samples.
Furthermore, they applied TR512-peptide in the signal enrichment, thus the novel detection strategy could offer an inexpensive, rapid and portable solution for areas with limited access to standard diagnosis laboratories.
The study was supported by the CAS Scientific Instruments and Equipment Development Program. (Text by ZHU Mingzhen)