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  English.dicp.cas.cn    Posted:2016-12-10
Biochemical Fractionation Strategy for Analysis of Protein Complexes Conserved across Metazoan

Time:9:00 am, Dec. 10th of 2016

Location:Academic Report Hall of Department of Biotechnology

Reporter:

Prof. Cuihong Wan obtained her Bachelor of Engineering degree from Huazhong University of Science and Technology in June of 2004. In July of 2009, she obtained her Ph.D. degree from Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. From October of 2009 to July of 2014, she carried out postdoctoral research in University of Toronto. From October of 2014 to June of 2015, she worked in University of Texas at Austin as research fellow. From October of 2015 to now, she worked in College of life sciences, Central China Normal University as professor and vice-dean.

Her research is focused on following areas: Landscape of protein complexes conserved across blue-green algae species; Drought resistant related protein complexes in Nostocflagellifor me; Proteomics and Mass Spectrometry method development, including high-throughput protein complexes identification, large-scale MS data analysis, protein-protein interaction data analysis, protein posttranslational modification and drug screening.

Abstract:

Knowledge of mutilprotein complexes is essential for understanding of biological processes, but their phylogenetic distribution across species, especially large-scale network mapping, is a challenge. Previous cross-species interactome comparisons, based on experimental data obtained from different sources and methods, typically show limited overlap. We examined comprehensive and accurate map of protein complexes common to metazoan by using a standardized approach, biochemical fractionation followed by quantitative mass spectrometry, to multiple species in parallel. In addition to human, 8 species were selected based on broad relevance as model organisms and breadth spanning a billion years of evolutionary divergence. We identified and quantified 13,386 protein orthologs across 6,387 fractions obtained from 69 different experiments, and final generate an interaction network consists of 16,655 high-confidenceco-complex interactions in human. Half of the predicted protein-protein interactions were supported by direct biochemical evidence in 3 or more input species, and half interactions have never been reported in any of five input species or yeast. Our study reveals the core physical underpinnings of the basic macromolecular machinery broadly relevant to metazoan physiology, development and evolution. The reconstructed interactome will provide rich functional insights into the fundamental molecular organization and evolutionary plasticity of animal cells.

Contact:Prof. Lihua Zhang of group 1810(phone: 9720)

 

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