Lecture: Reprogramming Saccharomyces Cerevisiae Metabolism for Efficient Production of Fatty Acid Derived Chemicals
Time: July 31, 2017, 2:00 pm Venue: Conference Room in Biotechnology Building Lecturer: YU Tao, Chalmers University of Technology, Sweden
Abstract: Microbial synthesis is a feasible route to achieve sustainable supply of fuels and chemicals. This talk first describes microbial production of (docosanol, C22H46O) in Saccharomyces cerevisiae by systematic reconstruction of fatty acid elongation system and expression of heterologous mycobacterial fatty acid synthases, to control chain length for synthesis of long chain fatty acid (C22-26). were achieved by the screening of fatty acid reductase, Through the reconstruction of synthetic pathway and dynamic control metabolic pathways, the engineered yeast produced p to 83.5 mg/L docosanol was in shake flask with unpaired cell growth. The second part of the work describes the modeling the oil producing mechanism of oleaginous yeast. We systematically rewrote the primary metabolism of S. cerevisiae, including enhancing the citric acid lyase, which was the main node of the mitochondrial pathway to increase the supply of precursor, increasing the phosphate pentose pathway for increased NADPH supply, re-directing the metabolic flux in the distribution of energy ATP and precursor supply. After the establishing the new "sugar - oil" metabolic model and optimizing the fermentation strategy, we achieved efficient synthesis of free fatty acids (34 g/L). Ultimately, the reprogramming of S. cerevisiae to oleaginous yeast is succeed by reprogramming the old S. cerevisiae "sugar-alcohol" metabolism with the aid of adaptive evolution.
Introduction: YU Tao, Ph.D., received a bachelor's degree in Shandong University in 2008. He received his Ph.D. degree from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences in 2014 with the main research interest in stem cells and reprogramming, abnormal protein degradation and cancer. From 2015 to now, he worked as a postdoctoral fellow in Jens Nielsen laboratory at the Chalmers University of Technology, Sweden, mainly engaged in construction of microbial cell factory for production of fatty acid derived of biofuels and chemicals. Representative work was published in Cell Research, Nature Communications, Journal of Molecular Cell Biology and Biotechnology and Bioengineering.
Contact: ZHOU Yongjin Phone: 84771060
DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACADEMY OF SCIENCES